Sinusoidal voltage protocols for rapid characterisation of ion channel kinetics.

ABSTRACT:

Key points

Ion current kinetics are commonly represented by current-voltage relationships, time constant-voltage relationships and subsequently mathematical models fitted to these. These experiments take substantial time, which means they are rarely performed in the same cell. Rather than traditional square-wave voltage clamps, we fitted a model to the current evoked by a novel sum-of-sinusoids voltage clamp that was only 8 s long. Short protocols that can be performed multiple times within a single cell will offer many new opportunities to measure how ion current kinetics are affected by changing conditions. The new model predicts the current under traditional square-wave protocols well, with better predictions of underlying currents than literature models. The current under a nov

SUBMITTER: Beattie KA 

PROVIDER: S-EPMC5978315 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature