Development of a micromanipulation method for single cell isolation of prokaryotes and its application in food safety.

ABSTRACT: For nearly a century, conventional microbiological methods have been standard practice for detecting and identifying pathogens in food. Nevertheless, the microbiological safety of food has improved and various rapid methods have been developed to overcome the limitations of conventional methods. Alternative methods are expected to detect low cell numbers, since the presence in food of even a single cell of a pathogenic organism may be infectious. With respect to low population levels, the performance of a detection method is assessed by producing serial dilutions of a pure bacterial suspension to inoculate representative food matrices with highly diluted bacterial cells (fewer than 10 CFU/ml). The accuracy of data obtained by multiple dilution techniques is not certain and does not exclude

SUBMITTER: Hohnadel M 

PROVIDER: S-EPMC5979034 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature