Metabolomics and Transcriptomics Identify Multiple Downstream Targets of Paraburkholderia phymatum σ⁵⁴ During Symbiosis with Phaseolus vulgaris.

ABSTRACT: RpoN (or σ⁵⁴) is the key sigma factor for the regulation of transcription of nitrogen fixation genes in diazotrophic bacteria, which include α- and β-rhizobia. Our previous studies showed that an rpoN mutant of the β-rhizobial strain Paraburkholderia phymatum STM815^T formed root nodules on Phaseolus vulgaris cv. Negro jamapa, which were unable to reduce atmospheric nitrogen into ammonia. In an effort to further characterize the RpoN regulon of P. phymatum, transcriptomics was combined with a powerful metabolomics approach. The metabolome of P. vulgaris root nodules infected by a P. phymatumrpoN Fix^- mutant revealed statistically significant metabolic changes compared to wild-type Fix⁺ nodules, including reduc

SUBMITTER: Lardi M 

PROVIDER: S-EPMC5979394 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature