Inhibition of yes-associated protein suppresses brain metastasis of human lung adenocarcinoma in a murine model.

ABSTRACT: Yes-associated protein (YAP) is a main mediator of the Hippo pathway and promotes cancer development and progression in human lung cancer. We sought to determine whether inhibition of YAP suppresses metastasis of human lung adenocarcinoma in a murine model. We found that metastatic NSCLC cell lines H2030-BrM3(K-ras^G12C mutation) and PC9-BrM3 (EGFR^Δexon19 mutation) had a significantly decreased p-YAP(S127)/YAP ratio compared to parental H2030 (K-ras^G12C mutation) and PC9 (EGFR^Δexon19 mutation) cells (P < .05). H2030-BrM3 cells had significantly increased YAP mRNA and expression of Hippo downstream genes CTGF and CYR61 compared to parental H2030 cells (P < .05). Inhibition of YAP by short hairpin RNA (shRNA) and small interfering RNA (siRNA) signif

SUBMITTER: Hsu PC 

PROVIDER: S-EPMC5980132 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature