Restricted T cell receptor repertoire in CLL-like monoclonal B cell lymphocytosis and early stage CLL.

ABSTRACT: Analysis of the T cell receptor (TR) repertoire of chronic lymphocytic leukemia-like monoclonal B cell lymphocytosis (CLL-like MBL) and early stage CLL is relevant for understanding the dynamic interaction of expanded B cell clones with bystander T cells. Here we profiled the T cell receptor β chain (TRB) repertoire of the CD4⁺ and CD8⁺ T cell fractions from 16 CLL-like MBL and 13 untreated, Binet stage A/Rai stage 0 CLL patients using subcloning analysis followed by Sanger sequencing. The T cell subpopulations of both MBL and early stage CLL harbored restricted TRB gene repertoire, with CD4⁺ T cell clonal expansions whose frequency followed the numerical increase of clonal B cells. Longitudinal analysis in MBL cases revealed clonal persistence, alluding to

SUBMITTER: Blanco G 

PROVIDER: S-EPMC5980379 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature