Cellular immunotherapy on primary multiple myeloma expanded in a 3D bone marrow niche model.

ABSTRACT: Bone marrow niches support multiple myeloma, providing signals and cell-cell interactions essential for disease progression. A 3D bone marrow niche model was developed, in which supportive multipotent mesenchymal stromal cells and their osteogenic derivatives were co-cultured with endothelial progenitor cells. These co-cultured cells formed networks within the 3D culture, facilitating the survival and proliferation of primary CD138⁺ myeloma cells for up to 28 days. During this culture, no genetic drift was observed within the genomic profile of the primary myeloma cells, indicating a stable outgrowth of the cultured CD138⁺ population. The 3D bone marrow niche model enabled testing of a novel class of engineered immune cells, so called TEGs (αβT cells engineered to exp

SUBMITTER: Braham MVJ 

PROVIDER: S-EPMC5980416 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature