P2Y₁₂ receptor modulation of ADP-evoked intracellular Ca²⁺ signalling in THP-1 human monocytic cells.

ABSTRACT:

Background and purpose

The G_i -coupled, ADP-activated P2Y₁₂ receptor is well characterized as playing a key role in platelet activation via crosstalk with the P2Y₁ receptor in ADP-evoked intracellular Ca²⁺ responses. However, there is limited knowledge on the role of P2Y₁₂ receptors in ADP-evoked Ca²⁺ responses in other blood cells. Here, we investigated the role of P2Y₁₂ receptor activation in the modulation of ADP-evoked Ca²⁺ responses in human THP-1 monocytic cells.

Experimental approach

A combination of intracellular Ca²⁺ measurements, RT-PCR, immunocytochemistry, leukocyte isolation and siRNA-mediated gene knockdown were used to identify the role of P2Y₁₂ recept

SUBMITTER: Micklewright JJ 

PROVIDER: S-EPMC5980558 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature