Modifications generated by fast photochemical oxidation of proteins reflect the native conformations of proteins.

ABSTRACT: Hydroxyl radical footprinting (HRF) is a nonspecific protein footprinting method that has been increasingly used in recent years to analyze protein structure. The method oxidatively modifies solvent accessible sites in proteins, which changes upon alterations in the protein, such as ligand binding or a change in conformation. For HRF to provide accurate structural information, the method must probe the native structure of proteins. This requires careful experimental controls since an abundance of oxidative modifications can induce protein unfolding. Fast photochemical oxidation of proteins (FPOP) is a HRF method that generates hydroxyl radicals via photo-dissociation of hydrogen peroxide using an excimer laser. The addition of a radical scavenger to the FPOP reaction reduces the lifetime o

SUBMITTER: Chea EE 

PROVIDER: S-EPMC5980583 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature