Ca2+/calmodulin-dependent protein kinase II and Dimethyl Sulfoxide affect the sealing frequencies of transected hippocampal neurons.

ABSTRACT: Traumatic injury often results in axonal severance, initiating obligatory Wallerian degeneration of distal segments, whereas proximal segments often survive. Calcium ion (Ca²⁺ ) influx at severed proximal axonal ends activates pathways that can induce apoptosis. However, this same Ca²⁺ -influx also activates multiple parallel pathways that seal the plasmalemma by inducing accumulation and fusion of vesicles at the lesion site that reduce Ca²⁺ -influx and enhance survival. We examined whether various inhibitors of Ca²⁺ /calmodulin-dependent protein kinases (CaMKs), and/or dimethyl sulfoxide (DMSO), a common solvent for biologically active substances, affected the ability of a hippocampal-derived neuronal cell line (B104 cells) to seal membrane dam

SUBMITTER: Poon AD 

PROVIDER: S-EPMC5980716 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature