Theory for the Liquid-Liquid Phase Separation in Aqueous Antibody Solutions.

ABSTRACT: This study presents the theory for liquid-liquid phase separation for systems of molecules modeling monoclonal antibodies. Individual molecule is depicted as an assembly of seven hard spheres, organized to mimic the Y-shaped antibody. We consider the antibody-antibody interactions either through Fab, Fab' (two Fab fragments may be different), or Fc domain. Interaction between these three domains of the molecule (hereafter denoted as A, B, and C, respectively) is modeled by a short-range square-well attraction. To obtain numerical results for the model under study, we adapt Wertheim's thermodynamic perturbation theory. We use this model to calculate the liquid-liquid phase separation curve and the second virial coefficient B2. Various interaction scenarios are examined to see how the streng

SUBMITTER: Kastelic M 

PROVIDER: S-EPMC5980754 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature