Compensation of Signal Spillover in Suspension and Imaging Mass Cytometry.

ABSTRACT: The advent of mass cytometry increased the number of parameters measured at the single-cell level while decreasing the extent of crosstalk between channels relative to dye-based flow cytometry. Although reduced, spillover still exists in mass cytometry data, and minimizing its effect requires considerable expert knowledge and substantial experimental effort. Here, we describe a novel bead-based compensation workflow and R-based software that estimates and corrects for interference between channels. We performed an in-depth characterization of the spillover properties in mass cytometry, including limitations defined by the linear range of the mass cytometer and the reproducibility of the spillover over time and across machines. We demonstrated the utility of our method in suspension and ima

SUBMITTER: Chevrier S 

PROVIDER: S-EPMC5981006 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature