Inhibitory effects of Myricetin derivatives on curli-dependent biofilm formation in Escherichia coli.

ABSTRACT: Biofilms are well-organised communities of microbes embedded in a self-produced extracellular matrix (e.g., curli amyloid fibers) and are associated with chronic infections. Therefore, development of anti-biofilm drugs is important to combat with these infections. Previously, we found that flavonol Myricetin inhibits curli-dependent biofilm formation by Escherichia coli (IC₅₀ = 46.2 μM). In this study, we tested activities of seven Myricetin-derivatives to inhibit biofilm formation by E. coli K-12 in liquid culture. Among them, only Epigallocatechin gallate (EGCG), a major catechin in green tea, inhibited biofilm formation of K-12 (IC₅₀ = 5.9 μM) more efficiently than Myricetin. Transmission electron microscopy and immunoblotting analyses demonstrated that EGCG preven

SUBMITTER: Arita-Morioka KI 

PROVIDER: S-EPMC5981455 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature