Electron transfer in an acidophilic bacterium: interaction between a diheme cytochrome and a cupredoxin.

ABSTRACT: Acidithiobacillus ferrooxidans, a chemolithoautotrophic Gram-negative bacterium, has a remarkable ability to obtain energy from ferrous iron oxidation at pH 2. Several metalloproteins have been described as being involved in this respiratory chain coupling iron oxidation with oxygen reduction. However, their properties and physiological functions remain largely unknown, preventing a clear understanding of the global mechanism. In this work, we focus on two metalloproteins of this respiratory pathway, a diheme cytochrome c₄ (Cyt c₄) and a green copper protein (AcoP) of unknown function. We first demonstrate the formation of a complex between these two purified proteins, which allows homogeneous intermolecular electron-transfer in solution. We then mimic the phys

SUBMITTER: Wang X 

PROVIDER: S-EPMC5982212 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature