Self-resistance guided genome mining uncovers new topoisomerase inhibitors from myxobacteria.

ABSTRACT: There is astounding discrepancy between the genome-inscribed production capacity and the set of known secondary metabolite classes from many microorganisms as detected under laboratory cultivation conditions. Genome-mining techniques are meant to fill this gap, but in order to favor discovery of structurally novel as well as bioactive compounds it is crucial to amend genomics-based strategies with selective filtering principles. In this study, we followed a self-resistance guided approach aiming at the discovery of inhibitors of topoisomerase, known as valid target in both cancer and antibiotic therapy. A common host self-defense mechanism against such inhibitors in bacteria is mediated by so-called pentapeptide repeat proteins (PRP). Genes encoding the biosynthetic machinery for productio

SUBMITTER: Panter F 

PROVIDER: S-EPMC5982219 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature