A High-Quality Reference Genome for the Invasive Mosquitofish Gambusia affinis Using a Chicago Library.

ABSTRACT: The western mosquitofish, Gambusia affinis, is a freshwater poecilid fish native to the southeastern United States but with a global distribution due to widespread human introduction. Gambusia affinis has been used as a model species for a broad range of evolutionary and ecological studies. We sequenced the genome of a male G. affinis to facilitate genetic studies in diverse fields including invasion biology and comparative genetics. We generated Illumina short read data from paired-end libraries and in vitro proximity-ligation libraries. We obtained 54.9× coverage, N50 contig length of 17.6 kb, and N50 scaffold length of 6.65 Mb. Compared to two other species in the Poeciliidae family, G. affinis has slightly fewer genes that have shorter total, exon, an

SUBMITTER: Hoffberg SL 

PROVIDER: S-EPMC5982815 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature