Modeling transcriptional activation changes to Gal4 variants via structure-based computational mutagenesis.

ABSTRACT: As a DNA binding transcriptional activator, Gal4 promotes the expression of genes responsible for galactose metabolism. The Gal4 protein from Saccharomyces cerevisiae (baker's yeast) has become a model for studying eukaryotic transcriptional activation in general because its regulatory properties mirror those of several eukaryotic organisms, including mammals. Given the availability of a crystallographic structure for Gal4, here we implement an in silico mutagenesis technique that makes use of a four-body knowledge-based energy function, in order to empirically quantify the structural impacts associated with single residue substitutions on the Gal4 protein. These results were used to examine the structure-function relationship in Gal4 based on a recently published experimenta

SUBMITTER: Masso M 

PROVIDER: S-EPMC5983003 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature