Age-dependent glycosylation of the sodium taurocholate cotransporter polypeptide: From fetal to adult human livers.

ABSTRACT: Sodium taurocholate cotransporter polypeptide (NTCP), mainly expressed on the sinusoidal membrane of hepatocytes, is one of the major transporters responsible for liver bile acid (BA) re-uptake. NTCP transports conjugated BA from the blood into hepatocytes and is crucial for correct enterohepatic circulation. Studies have shown that insufficient hepatic clearance of BA correlates with elevated serum BA in infants younger than 1 year of age. In the current study, we investigated human NTCP messenger RNA and protein expression by using reverse-transcription quantitative polymerase chain reaction and immunoblotting in isolated and cryopreserved human hepatocytes from two different age groups, below and above 1 year of age. Here, we show that NTCP messenger RNA expression is not modulated wher

SUBMITTER: Sargiacomo C 

PROVIDER: S-EPMC5983131 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature