Coexisting genomic aberrations associated with lymph node metastasis in breast cancer.

ABSTRACT: Single cancer cell-sequencing studies currently use randomly selected cells, limiting correlations among genomic aberrations, morphology, and spatial localization. We laser-captured microdissected single cells from morphologically distinct areas of primary breast cancer and corresponding lymph node metastasis and performed whole-exome or deep-target sequencing of more than 100 such cells. Two major subclones coexisted in different areas of the primary tumor, and the lymph node metastasis originated from a minor subclone in the invasive front of the primary tumor, with additional copy number changes, including chr8q gain, but no additional point mutations in driver genes. Lack of metastasis-specific driver events led us to assess whether other clonal and subclonal genomic aberrations preexi

SUBMITTER: Bao L 

PROVIDER: S-EPMC5983317 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature