Imaging pathological activities of human brain tissue in organotypic culture.

ABSTRACT:

Background

Insights into human brain diseases may emerge from tissue obtained after operations on patients. However techniques requiring transduction of transgenes carried by viral vectors cannot be applied to acute human tissue.

New method

We show that organotypic culture techniques can be used to maintain tissue from patients with three different neurological syndromes for several weeks in vitro. Optimized viral vector techniques and promoters for transgene expression are described.

Results

Region-specific differences in neuronal form, firing pattern and organization as well as pathological activities were maintained over 40-50 days in culture. Both adeno-associated virus and lentivirus based vectors were persistently expressed from ∼10 days after application, provi

SUBMITTER: Le Duigou C 

PROVIDER: S-EPMC5983351 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature