Hyaluronic Acid Influence on Normal and Osteoarthritic Tissue-Engineered Cartilage.

ABSTRACT: The aim of this study is to identify gene expression profiles associated with hyaluronic acid (HA) treatment of normal and osteoarthritis (OA)-like tissue-engineered cartilage. 3D cartilage micromasses were treated with tumour necrosis factor-α (TNF-α) (OA-inducer) and/or HA for 7 days. Viability was examined by PI/FDA staining. To document extracellular matrix (ECM) formation, glycosaminoglycans (GAG) were stained with Safranin-O and cartilage-specific type II collagen was detected immunohistochemically. Genome-wide gene expression was determined using microarray analysis. Normal and OA-like micromasses remained vital and showed a spherical morphology and homogenous cell distribution regardless of the treatment. There was no distinct difference in immunolabeling for type II collagen. Safr

SUBMITTER: Hemmati-Sadeghi S 

PROVIDER: S-EPMC5983669 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature