Single-Cell Chromatin Modification Profiling Reveals Increased Epigenetic Variations with Aging.

ABSTRACT: Post-translational modifications of histone proteins and exchanges of histone variants of chromatin are central to the regulation of nearly all DNA-templated biological processes. However, the degree and variability of chromatin modifications in specific human immune cells remain largely unknown. Here, we employ a highly multiplexed mass cytometry analysis to profile the global levels of a broad array of chromatin modifications in primary human immune cells at the single-cell level. Our data reveal markedly different cell-type- and hematopoietic-lineage-specific chromatin modification patterns. Differential analysis between younger and older adults shows that aging is associated with increased heterogeneity between individuals and elevated cell-to-cell variability in chromatin modification

SUBMITTER: Cheung P 

PROVIDER: S-EPMC5984186 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature