An engineered fatty acid synthase combined with a carboxylic acid reductase enables de novo production of 1-octanol in Saccharomyces cerevisiae.

ABSTRACT:

Background

The ideal biofuel should not only be a regenerative fuel from renewable feedstocks, but should also be compatible with the existing fuel distribution infrastructure and with normal car engines. As the so-called drop-in biofuel, the fatty alcohol 1-octanol has been described as a valuable substitute for diesel and jet fuels and has already been produced fermentatively from sugars in small amounts with engineered bacteria via reduction of thioesterase-mediated premature release of octanoic acid from fatty acid synthase or via a reversal of the β-oxidation pathway.

Results

The previously engineered short-chain acyl-CoA producing yeast Fas1^R1834K/Fas2 fatty acid synthase variant was expressed together with carboxylic acid reductase from Mycobacterium mar

SUBMITTER: Henritzi S 

PROVIDER: S-EPMC5984327 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature