Module and individual domain deletions of NRPS to produce plipastatin derivatives in Bacillus subtilis.

ABSTRACT:

Background

Plipastatin, an antifungal lipopeptide, is synthesized by a non-ribosomal peptide synthetase (NRPS) in Bacillus subtilis. However, little information is available on the combinatorial biosynthesis strategies applied in plipastatin biosynthetic pathway. In this study, we applied module or individual domain deletion strategies to engineer the plipastatin biosynthetic pathway, and investigated the effect of deletions on the plipastatin assembly line, as well as revealed the synthetic patterns of novel lipopeptides.

Results

Module deletion inactivated the entire enzyme complex, whereas individual domain (A/T domain) deletion within module 7 truncated the assembly line, resulting in truncated linear hexapeptides (C_16~17β-OHFA-Glu-Orn-Tyr-Thr-Glu-Ala/Val). In

SUBMITTER: Gao L 

PROVIDER: S-EPMC5984369 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature