Reconstruction of an SSR-based Magnaporthe oryzae physical map to locate avirulence gene AvrPi12.

ABSTRACT:

Background

Pathogen avirulence (Avr) genes can evolve rapidly when challenged by the widespread deployment of host genes for resistance. They can be effectively isolated by positional cloning provided a robust and well-populated genetic map is available.

Results

An updated, SSR-based physical map of the rice blast pathogen Magnaporthe oryzae (Mo) has been constructed based on 116 of the 120 SSRs used to assemble the last map, along with 18 newly developed ones. A comparison between the two versions of the map has revealed an altered marker content and order within most of the Mo chromosomes. The avirulence gene AvrPi12 was mapped in a population of 219 progeny derived from a cross between the two Mo isolates CHL42 and CHL357. A bulked segregant analysis indicated that the ge

SUBMITTER: Li T 

PROVIDER: S-EPMC5984427 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature