The genome-wide rate and spectrum of spontaneous mutations differ between haploid and diploid yeast.

ABSTRACT: By altering the dynamics of DNA replication and repair, alternative ploidy states may experience different rates and types of new mutations, leading to divergent evolutionary outcomes. We report a direct comparison of the genome-wide spectrum of spontaneous mutations arising in haploids and diploids following a mutation-accumulation experiment in the budding yeast Saccharomyces cerevisiae Characterizing the number, types, locations, and effects of thousands of mutations revealed that haploids were more prone to single-nucleotide mutations (SNMs) and mitochondrial mutations, while larger structural changes were more common in diploids. Mutations were more likely to be detrimental in diploids, even after accounting for the large impact of structural changes, contrary to the prediction

SUBMITTER: Sharp NP 

PROVIDER: S-EPMC5984525 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature