Elastic coupling power stroke mechanism of the F₁-ATPase molecular motor.

ABSTRACT: The angular velocity profile of the 120° F₁-ATPase power stroke was resolved as a function of temperature from 16.3 to 44.6 °C using a Δμ_ATP = -31.25 k_BT at a time resolution of 10 μs. Angular velocities during the first 60° of the power stroke (phase 1) varied inversely with temperature, resulting in negative activation energies with a parabolic dependence. This is direct evidence that phase 1 rotation derives from elastic energy (spring constant, κ = 50 k_BT·rad^-2). Phase 2 of the power stroke had an enthalpic component indicating that additional energy input occurred to enable the γ-subunit to overcome energy stored by the spring after rotating beyond its 34° equilibrium position. The correlation between t

SUBMITTER: Martin JL 

PROVIDER: S-EPMC5984535 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature