Analysis of HSP27 and the Autophagy Marker LC3B⁺ Puncta Following Preoperative Chemotherapy Identifies High-Risk Osteosarcoma Patients.

ABSTRACT: Chemotherapy-induced autophagy is a proposed mechanism of chemoresistance and potential therapeutic target in osteosarcoma. We evaluated heat shock protein 27 (HSP27) and autophagy-related proteins as predictors of pathologic treatment response and prognostic markers among osteosarcoma patients who received standard chemotherapy. We analyzed 394 tumor specimens (pre-treatment, post-treatment, and metastases) from 260 osteosarcoma patients by immunohistochemistry for cytoplasmic light chain 3B (LC3B)-positive puncta, sequestosome 1 (SQSTM1), high mobility group box 1 (HMGB1), and HSP27 expression. The staining percentage and intensity for each marker were scored and the extent to which marker expression was correlated with pathologic response, relapse-free survival (RFS), and overall surviv

SUBMITTER: Livingston JA 

PROVIDER: S-EPMC5984702 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature