Stage-dependent piRNAs in chicken implicated roles in modulating male germ cell development.

ABSTRACT:

Background

The PIWI/piRNA pathway is a conserved machinery important for germ cell development and fertility. This piRNA-guided molecular machinery is best known for repressing derepressed transposable elements (TE) during epigenomic reprogramming. The extent to which piRNAs are involved in modulating transcripts beyond TEs still need to be clarified, and it may be a stage-dependent event. We chose chicken germline as a study model because of the significantly lower TE complexity in the chicken genome compared to mammalian species.

Results

We generated high-confidence piRNA candidates in various stages across chicken germline development by 3'-end-methylation-enriched small RNA sequencing and in-house bioinformatics analysis. We observed a significant developmental stage-dep

SUBMITTER: Chang KW 

PROVIDER: S-EPMC5984780 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature