DNA assembly with error correction on a droplet digital microfluidics platform.

ABSTRACT:

Background

Custom synthesized DNA is in high demand for synthetic biology applications. However, current technologies to produce these sequences using assembly from DNA oligonucleotides are costly and labor-intensive. The automation and reduced sample volumes afforded by microfluidic technologies could significantly decrease materials and labor costs associated with DNA synthesis. The purpose of this study was to develop a gene assembly protocol utilizing a digital microfluidic device. Toward this goal, we adapted bench-scale oligonucleotide assembly methods followed by enzymatic error correction to the Mondrian™ digital microfluidic platform.

Results

We optimized Gibson assembly, polymerase chain reaction (PCR), and enzymatic error correction reactions in a single protocol

SUBMITTER: Khilko Y 

PROVIDER: S-EPMC5984785 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature