Effect of H-Bond Donor Lipids on Phosphatidylinositol-3,4,5-Trisphosphate Ionization and Clustering.

ABSTRACT: The phosphoinositide, phosphatidylinositol-3,4,5-trisphosphate (PI(3,4,5)P₃), is a key signaling lipid in the inner leaflet of the cell plasma membrane, regulating diverse signaling pathways including cell growth and migration. In this study we investigate the impact of the hydrogen-bond donor lipids phosphatidylethanolamine (PE) and phosphatidylinositol (PI) on the charge and phase behavior of PI(3,4,5)P₃. PE and PI can interact with PI(3,4,5)P₃ through hydrogen-bond formation, leading to altered ionization behavior and charge distribution within the PI(3,4,5)P₃ headgroup. We quantify the altered PI(3,4,5)P₃ ionization behavior using a multistate ionization model to obtain micro-pK_a values for the ionization of each phospha

SUBMITTER: Graber ZT 

PROVIDER: S-EPMC5984899 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature