Impact of Tissue Factor Localization on Blood Clot Structure and Resistance under Venous Shear.

ABSTRACT: The structure and growth of a blood clot depend on the localization of tissue factor (TF), which can trigger clotting during the hemostatic process or promote thrombosis when exposed to blood under pathological conditions. We sought to understand how the growth, structure, and mechanical properties of clots under flow are shaped by the simultaneously varying TF surface density and its exposure area. We used an eight-channel microfluidic device equipped with a 20- or 100-μm-long collagen surface patterned with lipidated TF of surface densities ∼0.1 and ∼2 molecules/μm². Human whole blood was perfused at venous shear, and clot growth was continually measured. Using our recently developed computational model of clot formation, we performed simulations to gain insights into the clot

SUBMITTER: Govindarajan V 

PROVIDER: S-EPMC5984989 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature