Motor Reattachment Kinetics Play a Dominant Role in Multimotor-Driven Cargo Transport.

ABSTRACT: Kinesin-based cargo transport in cells frequently involves the coordinated activity of multiple motors, including kinesins from different families that move at different speeds. However, compared to the progress at the single-molecule level, mechanisms by which multiple kinesins coordinate their activity during cargo transport are poorly understood. To understand these multimotor coordination mechanisms, defined pairs of kinesin-1 and kinesin-2 motors were assembled on DNA scaffolds and their motility examined in vitro. Although less processive than kinesin-1 at the single-molecule level, addition of kinesin-2 motors more effectively amplified cargo run lengths. By applying the law of total expectation to cargo binding durations in ADP, the kinesin-2 microtubule reattachment rate was shown

SUBMITTER: Feng Q 

PROVIDER: S-EPMC5985011 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature