Targeting erythropoietin protects against proteinuria in type 2 diabetic patients and in zebrafish.

ABSTRACT:

Objective

Adult human kidneys produce erythropoietin (EPO), which regulates red blood cell formation; however, whether EPO also functions directly on kidney development and controls diabetic kidney disease remains unknown. Here we analyzed the role of EPO in kidney development and under hyperglycemic conditions in zebrafish and in humans.

Methods

Diabetic patients and respective controls were enrolled in two cohorts. Serum EPO level and urine protein change upon human EPO administration were then analyzed. Transient knockdown and permanent knockout of EPO and EPOR in renal TG(WT1B:EGFP) zebrafish were established using the morpholino technology and CRISPR/Cas9 technology. Zebrafish embryos were phenotypically analyzed using fluorescence microscopy, and functional assays were

SUBMITTER: She J 

PROVIDER: S-EPMC5985015 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature