Degradation of brown adipocyte purine nucleotides regulates uncoupling protein 1 activity.

ABSTRACT:

Objective

Non-shivering thermogenesis in mammalian brown adipose tissue depends on thermogenic uncoupling protein 1. Its activity is triggered by free fatty acids while purine nucleotides mediate inhibition. During activation, it is thought that free fatty acids overcome purine-mediated inhibition. We measured the cellular concentration and the release of purine nucleotide metabolites to uncover a possible role of purine nucleotide degradation in uncoupling protein 1 activation.

Methods

With mass spectrometry, purine nucleotide metabolites were quantified in cellular homogenates and supernatants of cultured primary brown adipocytes. We also determined oxygen consumption in response to a β-adrenergic agonist.

Results

Upon adrenergic activation, brown adipocytes decreas

SUBMITTER: Fromme T 

PROVIDER: S-EPMC5985227 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature