Prolyl carboxypeptidase in Agouti-related Peptide neurons modulates food intake and body weight.

ABSTRACT: OBJECTIVE:Prolyl carboxypeptidase (PRCP) plays a role in the regulation of energy metabolism by inactivating hypothalamic α-melanocyte stimulating hormone (α-MSH) levels. Although detected in the arcuate nucleus, limited PRCP expression has been observed in the arcuate POMC neurons, and its site of action in regulating metabolism is still ill-defined. METHODS:We performed immunostaining to assess the localization of PRCP in arcuate Neuropeptide Y/Agouti-related Peptide (NPY/AgRP) neurons. Hypothalamic explants were then used to assess the intracellular localization of PRCP and its release at the synaptic levels. Finally, we generated a mouse model to assess the role of PRCP in NPY/AgRP neurons of the arcuate nucleus in the regulation of metabolism. RESULTS:Here we show that PRCP is express

SUBMITTER: Bruschetta G 

PROVIDER: S-EPMC5985234 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature