Development qRT-PCR Protocol to Predict Strawberry Fusarium Wilt Occurrence.

ABSTRACT: Strawberry Fusarium wilt disease, caused by Fusarium oxysporum f. sp. fragariae, is the most devastating disease in strawberry production. The pathogen produces chlamydospores which tolerate against harsh environment, fungicide and survive for decades in soil. Development of detection and quantification techniques are regarded significantly in many soilborne pathogens to prevent damage from diseases. In this study, we improved specific-quantitative primers for F. oxysporum f. sp. fragariae to reveal correlation between the pathogen density and the disease severity. Standard curve r² value of the specific-quantitative primers for qRT-PCR and meting curve were over 0.99 and 80.5°C, respectively. Over pathogen 10⁵ cfu/g of soil was required to

SUBMITTER: Hong SW 

PROVIDER: S-EPMC5985642 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature