Reciprocal signalling by Notch-Collagen V-CALCR retains muscle stem cells in their niche.

ABSTRACT: The cell microenvironment, which is critical for stem cell maintenance, contains both cellular and non-cellular components, including secreted growth factors and the extracellular matrix^1-3. Although Notch and other signalling pathways have previously been reported to regulate quiescence of stem cells^4-9, the composition and source of molecules that maintain the stem cell niche remain largely unknown. Here we show that adult muscle satellite (stem) cells in mice produce extracellular matrix collagens to maintain quiescence in a cell-autonomous manner. Using chromatin immunoprecipitation followed by sequencing, we identified NOTCH1/RBPJ-bound regulatory elements adjacent to specific collagen genes, the expression of which is deregulated in Notch-mutant mice. Moreover,

SUBMITTER: Baghdadi MB 

PROVIDER: S-EPMC5985950 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature