Next generation calmodulin affinity purification: Clickable calmodulin facilitates improved protein purification.

ABSTRACT: As the proteomics field continues to expand, scientists are looking to integrate cross-disciplinary tools for studying protein structure, function, and interactions. Protein purification remains a key tool for many characterization studies. Calmodulin (CaM) is a calcium-binding messenger protein with over a hundred downstream binding partners, and is involved in a host of physiological processes, from learning and memory to immune and cardiac function. To facilitate biophysical studies of calmodulin, researchers have designed a site-specific labeling process for use in bioconjugation applications while maintaining high levels of protein activity. Here, we present a platform for selective conjugation of calmodulin directly from clarified cell lysates under bioorthogonal reaction conditions.

SUBMITTER: Fraseur JG 

PROVIDER: S-EPMC5986150 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature