The F domain of estrogen receptor α is involved in species-specific, tamoxifen-mediated transactivation.

ABSTRACT: Estrogen receptor α (ERα) is a major transducer of estrogen-mediated physiological signals. ERα is a member of the nuclear receptor superfamily, which encompasses ligand-dependent transcription factors. The C terminus of nuclear receptors, termed the F domain, is the least homologous region among the members of this family. The ERα F domain possesses 45 amino acids; however, its function remains unclear. We noticed that the homology of the F domains between mouse and human ERαs is remarkably lower (75.6% similarity) than that between the entire proteins (94.7% similarity). To assess the functionality of the ERα F domains, here we generated chimeric ERα expression constructs with mouse-human-exchanged F domains. Using cell-based in vitro assays, we analyzed the transcriptional coacti

SUBMITTER: Arao Y 

PROVIDER: S-EPMC5986198 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature