Engineering of a rough auxotrophic mutant Salmonella Typhimurium for effective delivery.

ABSTRACT: Live Salmonella vaccine vectors offer a remarkable platform for delivering immunogens and therapeutic molecules by mimicking natural intracellular infections; however, pre-existing anti-vector immunity can impede effective deployment. Measures to alleviate pre-existing immunity include the use of heterologous vectors, development of highly attenuated strain enabling greater payload, removal of major immunoreactive components from the vector, and/or augmentation of delivered antigens via increased presentation in antigen presenting cells. Here we report a Salmonella Typhimurium (ST) vector-JOL1800 that embodies these requisite properties. JOL1800 is a highly attenuated, auxotrophic, and O-antigen deficient rough-mutant strain. Heterologous bacterial and viral antigens were exp

SUBMITTER: Lalsiamthara J 

PROVIDER: S-EPMC5986645 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature