Cotransporter-mediated water transport underlying cerebrospinal fluid formation.

ABSTRACT: Cerebrospinal fluid (CSF) production occurs at a rate of 500 ml per day in the adult human. Conventional osmotic forces do not suffice to support such production rate and the molecular mechanisms underlying this fluid production remain elusive. Using ex vivo choroid plexus live imaging and isotope flux in combination with in vivo CSF production determination in mice, we identify a key component in the CSF production machinery. The Na⁺/K⁺/2Cl^- cotransporter (NKCC1) expressed in the luminal membrane of choroid plexus contributes approximately half of the CSF production, via its unusual outward transport direction and its unique ability to directly couple water transport to ion translocation. We thereby establish the concept of cotransport of water as a missin

SUBMITTER: Steffensen AB 

PROVIDER: S-EPMC5986890 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature