P62 is linked to mitophagy in oleic acid-induced adipogenesis in human adipose-derived stromal cells.

ABSTRACT:

Background

Obesity is closely related to the abnormal differentiation of adipocytes, which are subjected to high plasma levels of free fatty acids (FFAs). As the most abundant FFA in the bloodstream, oleic acid (OA) has the ability to induce adipogenic differentiation in human adipose-derived stromal cells (hADSCs). Recently, p62, an autophagy mediator, has been shown to play a role in obesity and adipose tissue metabolism. Therefore, the aim of this study was to investigate the roles of autophagy and mitochondrial function at different stages of OA (in combination with insulin and dexamethasone)-induced adipogenesis in hADSCs.

Methods

The hADSCs were incubated with OA, insulin, and dexamethasone after pretreatment with autophagy inhibitors or knockdown of p62 with shRNA. Th

SUBMITTER: Zeng R 

PROVIDER: S-EPMC5987550 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature