DamID profiling of dynamic Polycomb-binding sites in Drosophila imaginal disc development and tumorigenesis.

ABSTRACT:

Background

Tracking dynamic protein-chromatin interactions in vivo is key to unravel transcriptional and epigenetic transitions in development and disease. However, limited availability and heterogeneous tissue composition of in vivo source material impose challenges on many experimental approaches.

Results

Here we adapt cell-type-specific DamID-seq profiling for use in Drosophila imaginal discs and make FLP/FRT-based induction accessible to GAL driver-mediated targeting of specific cell lineages. In a proof-of-principle approach, we utilize ubiquitous DamID expression to describe dynamic transitions of Polycomb-binding sites during wing imaginal disc development and in a scrib tumorigenesis model. We identify Atf3 and Ets21C as novel Polycomb target genes involved in scrib

SUBMITTER: La Fortezza M 

PROVIDER: S-EPMC5987561 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature