Mosquito arbovirus survey in selected areas of Kenya: detection of insect-specific virus.

ABSTRACT:

Background

Many arboviral outbreaks have occurred in various locations in Kenya. Entomological surveys are suitable methods for revealing information about circulating arboviruses before human outbreaks are recognized. Therefore, mosquitoes were collected in Kenya to determine the distribution of arboviruses.

Methods

Various species of mosquitoes were sampled from January to July 2012 using several collection methods. Mosquito homogenates were directly tested by reverse transcription-polymerase chain reaction (RT-PCR) using various arbovirus-targeted primer pairs.

Results

We collected 12,569 mosquitoes. Although no human-related arboviruses were detected, Culex flavivirus (CxFV), an insect-specific arbovirus, was detected in 54 pools of 324 Culex quinquefasciatus</

SUBMITTER: Iwashita H 

PROVIDER: S-EPMC5987586 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature