Genome skimming herbarium specimens for DNA barcoding and phylogenomics.

ABSTRACT:

Background

The world's herbaria contain millions of specimens, collected and named by thousands of researchers, over hundreds of years. However, this treasure has remained largely inaccessible to genetic studies, because of both generally limited success of DNA extraction and the challenges associated with PCR-amplifying highly degraded DNA. In today's next-generation sequencing world, opportunities and prospects for historical DNA have changed dramatically, as most NGS methods are actually designed for taking short fragmented DNA molecules as templates.

Results

As a practical test of routine recovery of rDNA and plastid genome sequences from herbarium specimens, we sequenced 25 herbarium specimens up to 80 years old from 16 different Angiosperm families. Paired-end reads we

SUBMITTER: Zeng CX 

PROVIDER: S-EPMC5987614 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature