Laboratory testing and phylogenetic analysis during a mumps outbreak in Ontario, Canada.

ABSTRACT:

Background

In September 2009, a mumps outbreak originated in New York and spread to Northeastern USA and Canada. This study compares the performance of different diagnostic testing methods used in Ontario and describes molecular characteristics of the outbreak strain.

Methods

Between September 2009 and February 2010, specimens from suspect cases were submitted to Public Health Ontario Laboratory for mumps serology, culture and/or real-time reverse-transcriptase PCR (rRT-PCR) testing. rRT-PCR-positive specimens underwent genotyping at Canada's National Microbiology Laboratory. Whole genome sequencing was performed on four outbreak and three sporadic viral culture isolates.

Results

Six hundred ninety-eight patients had IgM serology testing, of which 255 (37%) had cultur

SUBMITTER: L'Huillier AG 

PROVIDER: S-EPMC5987625 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature