Engineering Saccharomyces cerevisiae for the production of the valuable monoterpene ester geranyl acetate.

ABSTRACT:

Background

Geranyl acetate is widely used in the fragrance and cosmetic industries, and thus has great economic value. However, plants naturally produce a mixture of hundreds of esters, and geranyl acetate is usually only present in trace amounts, which makes its economical extraction from plant sources practically impossible. As an ideal host for heterologous production of fragrance compound, the Saccharomyces cerevisiae has never been engineered to produce the esters, such as geranyl acetate.

Results

In this study, a heterologous geranyl acetate synthesis pathway was constructed in S. cerevisiae for the first time, and a titer of 0.63 mg/L geranyl acetate was achieved. By expressing an Erg20 mutant to divert carbon flux from FPP to GPP, the geranyl acetate production incre

SUBMITTER: Wu T 

PROVIDER: S-EPMC5987629 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature