Active and inactive β1 integrins segregate into distinct nanoclusters in focal adhesions.

ABSTRACT: Integrins are the core constituents of cell-matrix adhesion complexes such as focal adhesions (FAs) and play key roles in physiology and disease. Integrins fluctuate between active and inactive conformations, yet whether the activity state influences the spatial organization of integrins within FAs has remained unclear. In this study, we address this question and also ask whether integrin activity may be regulated either independently for each integrin molecule or through locally coordinated mechanisms. We used two distinct superresolution microscopy techniques, stochastic optical reconstruction microscopy (STORM) and stimulated emission depletion microscopy (STED), to visualize active versus inactive β1 integrins. We first reveal a spatial hierarchy of integrin organization with integrin

SUBMITTER: Spiess M 

PROVIDER: S-EPMC5987715 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature