PicW2 from Picea wilsonii: preparation, purification, crystallization and X-ray diffraction analysis.

ABSTRACT: Low temperature is a major limiting factor for plant growth and development. Dehydrin proteins are generally induced in response to low-temperature stress. In previous research, a full-length dehydrin gene, PicW2, was isolated from Picea wilsonii and its expression was associated with hardiness to cold. In order to gain insight into the mechanism of low-temperature tolerance by studying its three-dimensional crystal structure, prokaryotically expressed PicW2 dehydrin protein was purified using chitosan-affinity chromatography and gel filtration, and crystallized using the vapour-diffusion method. The crystal grew in a condition consisting of 0.1 M HEPES pH 8.0, 25%(w/v) PEG 3350 using 4 mg ml^-1 protein solution at 289 K. X-ray diffraction data were collected from a crystal at 10

SUBMITTER: Zhang B 

PROVIDER: S-EPMC5987745 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature