Ligand binding and activation properties of the purified bacterial cyclic nucleotide-gated channel SthK.

ABSTRACT: Cyclic nucleotide-modulated ion channels play several essential physiological roles. They are involved in signal transduction in photoreceptors and olfactory sensory neurons as well as pacemaking activity in the heart and brain. Investigations of the molecular mechanism of their actions, including structural and electrophysiological characterization, are restricted by the availability of stable, purified protein obtained from accessible systems. Here, we establish that SthK, a cyclic nucleotide-gated (CNG) channel from Spirochaeta thermophila, is an excellent model for investigating the gating of eukaryotic CNG channels at the molecular level. The channel has high sequence similarity with its eukaryotic counterparts and was previously reported to be activated by cyclic nucleotides i

SUBMITTER: Schmidpeter PAM 

PROVIDER: S-EPMC5987880 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature